Generator

Part:BBa_K3165048:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-16)


ccdB (L83S) under araBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 396


Design Notes

Codon optimised for E. coli with a core mutation at the 83rd amino acid converting Leu to Ser.


Source

The part has been derived from Professor Raghavan Varadarajan's Lab.

References